Development and Validation of an Indirect and Blocking ELISA for the Serological Diagnosis of African Swine Fever.

ABSTRACT: African swine fever (ASF) is an economically devastating viral disease of pigs caused by the ASF virus (ASFV). The rapid global spread of ASF has increased the demand for ASF diagnostics to be readily available and accessible. No commercial ASF enzyme-linked immunosorbent assay (ELISA) kits are manufactured and licensed in North America. Here, we report the development of two serological diagnostic assays, a blocking ELISA (bELISA) based on ASFV glycoprotein p54 and an indirect ELISA (iELISA) based on ASFV glycoproteins p54 and p72. The assays showed high sensitivity and specificity and detected anti-ASFV antibodies in serum samples from experimentally infected animals as early as 8 days post-infection. The two assays were produced commercially (AsurDx^™ bELISA and iELISA) and su

SUBMITTER: Onyilagha C 

PROVIDER: S-EPMC11597605 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature