Electrochemical Aptamer-Based Biosensor for Detecting Pap31, a Biomarker for Carrion's Disease.

ABSTRACT: Carrion's disease, caused by infection with the bacterium Bartonella bacilliformis (B. bacilliformis), is effectively treated with antibiotics, but reaches fatality rates of ~90% if untreated. Current diagnostic methods are limited, insufficiently sensitive, or require laboratory technology unavailable in endemic areas. Electrochemical aptamer-based (E-AB) biosensors provide a potential solution for this unmet need, as these biosensors are portable, sensitive, and can rapidly report the detection of small molecule targets. Here, we developed an E-AB biosensor to detect Pap31, a biomarker of Carrion's disease and an outer membrane protein in B. bacilliformis. We identified an aptamer with Pap31-specific binding affinity using a magnetic pull-down assay with magnetic bea

SUBMITTER: Silver K 

PROVIDER: S-EPMC11598354 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature