Novel Hybrid Catalysts of Cysteine Proteases Enhanced by Chitosan and Carboxymethyl Chitosan Micro- and Nanoparticles.

ABSTRACT: Micro- and nanoparticles of chitosan and carboxymethyl chitosan were synthesized, both with and without ascorbic acid. Methods were developed to form complexes between these micro- and nanoparticles and plant proteases-ficin, papain, and bromelain. It was demonstrated that the activity of cysteine protease complexes with carboxymethyl chitosan micro- and nanoparticles was higher compared to those with chitosan micro- and nanoparticles. Additionally, the complexes of ficin, papain, and bromelain with chitosan and carboxymethyl chitosan micro- and nanoparticles synthesized in the presence of ascorbic acid exhibited greater proteolytic activity than those formed with particles prepared without ascorbic acid. Molecular docking studies revealed that the amino acid residues of ficin, papain, and

SUBMITTER: Holyavka M 

PROVIDER: S-EPMC11598686 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature