A Novel and Rapid Selective Viral Genome Amplification and Sequencing Method for African Swine Fever Virus.

ABSTRACT: African swine fever virus (ASFV) is the etiological agent of African swine fever, a highly contagious hemorrhagic disease affecting both wild boars and domestic pigs with lethality rates up to 100%. Until now, the most effective measure to prevent an outbreak of ASFV was early detection. In this situation, whole genome sequencing (WGS) allows the gathering of detailed information about the identity and epidemiology of the virus. However, due to the large genome size and complex genome ends, WGS is challenging. Current WGS workflows require either elaborate enrichment methods or are based on tiled PCR approaches, which are susceptible to genetic differences between ASFV strains. To overcome this, we developed a novel approach for WGS of ASFV, using the Phi29 DNA polymerase-based multiple di

SUBMITTER: Licheri M 

PROVIDER: S-EPMC11598870 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature