The Transcriptional Program of <i>Staphylococcus aureus</i> Phage K Is Affected by a Host <i>rpoC</i> Mutation That Confers Phage K Resistance.

ABSTRACT: To better understand host-phage interactions and the genetic bases of phage resistance in a model system relevant to potential phage therapy, we isolated several spontaneous mutants of the USA300 S. aureus clinical isolate NRS384 that were resistant to phage K. Six of these had a single missense mutation in the host rpoC gene, which encodes the RNA polymerase β' subunit. To examine the hypothesis that mutations in the host RNA polymerase affect the transcription of phage genes, we performed RNA-seq analysis on total RNA samples collected from NRS384 wild-type (WT) and rpoC_G17D mutant cultures infected with phage K, at different timepoints after infection. Infection of the WT host led to a steady increase of phage transcription relative to the host. Our analy

SUBMITTER: Kongari R 

PROVIDER: S-EPMC11598898 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature