Reproductive Tract Mucus May Influence the Sex of Offspring in Cattle: Study in Cows That Have Repeatedly Calved Single-Sex Offspring.

ABSTRACT: This study aimed to investigate the selective effect of the reproductive tract mucus in cows that have consistently produced offspring of a single sex on X/Y spermatozoa. We collected mucus from the reproductive tract of cows that had given calvings to offspring of the same sex, or alternated between sexes, for more than five consecutive calvings. We evaluated the pH of reproductive tract mucus. Subsequently, we conducted a spermatozoa penetration assay; the proportions of X and Y spermatozoa after penetration were then identified by dual TaqMan qPCR and flow cytometry. This was followed by in vitro fertilization and embryo sex determination experiments. Immediately afterwards, computer-aided spermatozoa analysis was employed to analyze the spermatozoa that had penetrated through different

SUBMITTER: Huang F 

PROVIDER: S-EPMC11598928 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature