Specific imaging of CD8 + T-Cell dynamics with a nanobody radiotracer against human CD8β.

ABSTRACT:

Purpose

While immunotherapy has revolutionized the oncology field, variations in therapy responsiveness limit the broad applicability of these therapies. Diagnostic imaging of immune cell, and specifically CD8⁺ T cell, dynamics could allow early patient stratification and result in improved therapy efficacy and safety. In this study, we report the development of a nanobody-based immunotracer for non-invasive SPECT and PET imaging of human CD8⁺ T-cell dynamics.

Methods

Nanobodies targeting human CD8β were generated by llama immunizations and subsequent biopanning. The lead anti-human CD8β nanobody was characterized on binding, specificity, stability and toxicity. The lead nanobody was labeled with technetium-99m, gallium-68 and copper-64 for non-invasive

SUBMITTER: De Groof TWM 

PROVIDER: S-EPMC11599301 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature