Metabolomics and ¹³C labelled glucose tracing to identify carbon incorporation into aberrant cell membrane glycans in cancer.

ABSTRACT: Cell membrane glycans contribute to immune recognition, signaling, and cellular adhesion and migration, and altered membrane glycosylation is a feature of cancer cells that contributes to cancer progression. The uptake and metabolism of glucose and other nutrients essential for glycan synthesis could underlie altered membrane glycosylation, but the relationship between shifts in nutrient metabolism and the effects on glycans have not been directly examined. We developed a method that combines stable isotope tracing with metabolomics to enable direct observations of glucose allocation to nucleotide sugars and cell-membrane glycans. We compared the glucose allocation to membrane glycans of two pancreatic cancer cell lines that are genetically identical but have differing energy requirements.

SUBMITTER: Reyes-Oliveras A 

PROVIDER: S-EPMC11599571 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature