Altered O-Glycans in stimulated whole saliva from patients with primary Sjogren's syndrome and non-pSS sicca.

ABSTRACT: To investigate if salivary O-linked glycans are altered in primary Sjögren's syndrome (pSS), and thus contributing to explain symptoms of oral dryness, and an impaired oral mucosal barrier function leading to changes in microbial metabolism and colonization by both pathogenic and commensal microorganisms and increased prevalence of oral diseases. O-linked oligosaccharides from stimulated whole saliva (SWS) samples from 24 patients with pSS, 38 patients with non-pSS sicca, and 23 healthy controls were analyzed using liquid chromatography mass spectrometer (LC-MS). Non-fractionated reduced and alkylated saliva was dot-blotted to PVDF-membrane and O-linked oligosaccharides were released using reductive beta-elimination. The 50 most abundant glycans were identified and their intensity compared

SUBMITTER: Kamounah S 

PROVIDER: S-EPMC11599585 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature