Multiomics analysis of Staphylococcus aureus ST239 strains resistant to virulent Herelleviridae phages.

ABSTRACT: In the context of the antimicrobial therapy crisis, the significance of studying and implementing alternative treatment methods, particularly phage therapy, is increasingly evident. This study aimed to investigate the resistance of clinical Staphylococcus aureus ST239 strains to Herelleviridae phages through comparative genomics, transcriptomics, and proteomics. Analysis of resistant and sensitive S. aureus strains showed that resistant strains form a separate cluster on the phylogenetic tree, suggesting unique genetic traits underlying their phage resistance. Further in-depth analysis of the resistant SA191 strain infected with Herelleviridae phage, compared to an uninfected control, unveiled significant changes in the transcription of 462 genes (271↑ 191↓) at 5 min and 504 genes (276↑ 22

SUBMITTER: Kornienko M 

PROVIDER: S-EPMC11599779 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature