TOMM40 regulates hepatocellular and plasma lipid metabolism via an LXR-dependent pathway.

ABSTRACT:

Objective

The gene encoding TOMM40 (Transporter of Outer Mitochondrial Membrane 40) is adjacent to that encoding APOE, which has a central role in lipid and lipoprotein metabolism. While human genetic variants near APOE and TOMM40 have been shown to be strongly associated with plasma lipid levels, a specific role for TOMM40 in lipid metabolism has not been established, and the present study was aimed at assessing this possibility.

Methods

TOMM40 was knocked down by siRNA in human hepatoma HepG2 cells, and effects on mitochondrial function, lipid phenotypes, and crosstalk between mitochondria, ER, and lipid droplets were examined. Additionally, hepatic and plasma lipid levels were measured in mice following shRNA-induced knockdown of Tomm40 shRNA.

Results

In HepG2 cell

SUBMITTER: Yang NV 

PROVIDER: S-EPMC11600064 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature