Native Cryo-Correlative Light and Synchrotron X-ray Fluorescence Imaging of Proteins and Essential Metals in Subcellular Neuronal Compartments.

ABSTRACT: Essential metals such as iron, copper, and zinc are required for a wide variety of biological processes. For example, they act as cofactors in many proteins, conferring enzymatic activity or structural stability. Interactions between metals and proteins are often difficult to characterize due to the low concentration of metals in biological tissues and the sometimes labile nature of the chemical bonds involved. To better understand the cellular functions of essential metals, we correlate protein localization, using fluorescence light microscopy (FLM), and metal distribution with synchrotron X-ray fluorescence (SXRF), a high-sensitivity and high-spatial-resolution technique for metal imaging. Both chemical imaging modalities are implemented under cryogenic conditions to preserve native cell

SUBMITTER: Ortega R 

PROVIDER: S-EPMC11600181 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature