Identification of a terpene synthase arsenal using long-read sequencing and genome assembly of Aspergillus wentii.

ABSTRACT:

Background

Fungi are talented producers of secondary metabolites with applications in the pharmaceutical and agrochemical sectors. Aspergillus wentii CBS 141173 has gathered research interest due to its ability to produce high-value norditerpenoid compounds, including anticancer molecules. In this study, we aimed to expand the genomic information available for A. wentii to facilitate the identification of terpenoid biosynthetic genes that may be involved in the production of bioactive molecules.

Results

Long-read genome sequencing of Aspergillus wentii CBS 141173 was conducted using Oxford Nanopore Technologies (ONT) MinION MK1C. In addition, paired-end stranded RNA-seq data from two time points, 7 days and 30 days, was used for functional annotation of the assembled genome.

SUBMITTER: Olumakaiye R 

PROVIDER: S-EPMC11600568 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature