DNA methylation analysis of peripheral blood mononuclear cells in diagnosing breast cancer from benign breast lesions.

ABSTRACT:

Background

With the increasing incidence of breast lesions, the differential diagnosis between benign lesions and breast cancer (BCa) has become a big challenge. Host peripheral blood mononuclear cells (PBMCs) could undergo changes in DNA methylation upon disease progression. However, the clinical value of DNA methylation of PBMCs in differentiating benign lesions and BCa is still unclear.

Methods

DNA of PBMCs was isolated and the methylation status of PBMCs in patients with BCa and benign breast nodules was detected by using Illumina Infinium methylation EPIC array. The specific methylation targets were validated by pyrosequencing, Targeted Bisulfite Sequencing Assay, and Multiplex Methylation PCR Assay(MMPA).

Results

cg26977936, cg23351954, and cg27209741 were valid

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC11600940 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature