Subretinal Amniotic Membrane Transplantation in a Porcine Model of Retinal Hole.

ABSTRACT:

Purpose

To investigate the histopathological changes following subretinal amniotic membrane transplantation in an in vivo porcine model of retinal holes.

Methods

Left eyes of 12 Danish Landrace pigs were vitrectomized under full anesthesia. A subretinal bleb was created before excising a retinal hole (1154-2934 µm) using a 23-gauge vitrector. The pigs underwent transplantation of human freeze-dried amniotic membrane into the subretinal space, with no tamponade applied. Optical coherence tomography and color fundus photography were performed just after surgery and at 2 and 4 weeks post-surgery. At the end of follow-up, the eyes were enucleated for hematoxylin and eosin staining and fluorescence immunohistochemistry, using antibodies against retinal glial cells and inner retin

SUBMITTER: Olufsen ME 

PROVIDER: S-EPMC11601133 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature