Small Extracellular Vesicle-Associated MiRNAs in Polarized Retinal Pigmented Epithelium.

ABSTRACT:

Purpose

Oxidative stress in the retinal pigmented epithelium (RPE) has been implicated in age-related macular degeneration by impacting endocytic trafficking, including the formation, content, and secretion of extracellular vesicles (EVs). Using our model of polarized primary porcine RPE (pRPE) cells under chronic subtoxic oxidative stress, we tested the hypothesis that RPE miRNAs packaged into EVs are secreted in a polarized manner and contribute to maintaining RPE homeostasis.

Methods

Small EVs (sEVs) enriched for exosomes were isolated from apical and basal conditioned media from pRPE cells grown for up to four weeks with or without low concentrations of hydrogen peroxide using two sEV isolation methods, leading to eight experimental groups. The sEV miRNA expression was p

SUBMITTER: Hernandez BJ 

PROVIDER: S-EPMC11601136 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature