UV-Induced DNA Repair Mechanisms and Their Effects on Mutagenesis and Culturability in <i>Escherichia coli</i>.

ABSTRACT: Mutagenic processes drive evolutionary progress, with ultraviolet (UV) radiation significantly affecting evolution. Despite extensive research on SOS response-mediated mutagenesis, UV-induced repair mechanisms remain complex, and their effects on cell survival and mutagenesis are not fully understood. We previously observed a near-perfect correlation between RecA-mediated SOS response and mutation levels in Escherichia coli following UV treatment. However, prolonged UV exposure caused transient non-culturability and impaired SOS-mediated mutagenesis. Using fluorescent reporters, flow cytometry, promoter-reporter assays, single-gene deletions, knockouts, and clonogenic assays, we found that excessive UV exposure disrupts cellular translation, reducing SOS gene expression, albeit with

SUBMITTER: Ghosh S 

PROVIDER: S-EPMC11601333 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature