Epistasis and cryptic QTL identified using modified bulk segregant analysis of copper resistance in budding yeast.

ABSTRACT: The contributions of genetic interactions to natural trait variation are challenging to estimate experimentally, as current approaches for detecting epistasis are often underpowered. Powerful mapping approaches such as bulk segregant analysis, wherein individuals with extreme phenotypes are pooled for genotyping, obscure epistasis by averaging over genotype combinations. To accurately characterize and quantify epistasis underlying natural trait variation, we have engineered strains of the budding yeast Saccharomyces cerevisiae to enable crosses where one parent's chromosome is fixed while the rest of the chromosomes segregate. These crosses allow us to use bulk segregant analysis to identify quantitative trait loci (QTL) whose effects depend on alleles on the fixed parental chromoso

SUBMITTER: Buzby C 

PROVIDER: S-EPMC11601411 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature