Interlaboratory comparison of standardised metabolomics and lipidomics analyses in human and rodent blood using the MxP^® Quant 500 kit.

ABSTRACT: Metabolomics and lipidomics are pivotal in understanding phenotypic variations beyond genomics. However, quantification and comparability of mass spectrometry (MS)-derived data are challenging. Standardised assays can enhance data comparability, enabling applications in multi-center epidemiological and clinical studies. Here we evaluated the performance and reproducibility of the MxP^® Quant 500 kit across 14 laboratories. The kit allows quantification of 634 different metabolites from 26 compound classes using triple quadrupole MS. Each laboratory analysed twelve samples, including human plasma and serum, lipaemic plasma, NIST SRM 1950, and mouse and rat plasma, in triplicates. 505 out of the 634 metabolites were measurable above the limit of detection in all laboratories, while

SUBMITTER: Zararsiz GE 

PROVIDER: S-EPMC11601468 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature