Anillin tunes contractility and regulates barrier function during Rho flare-mediated tight junction remodeling.

ABSTRACT: To preserve barrier function, cell-cell junctions must dynamically remodel during cell shape changes. We have previously described a rapid tight junction repair pathway characterized by local, transient activation of RhoA, termed 'Rho flares,' which repair leaks in tight junctions via promoting local actomyosin-mediated junction remodeling. In this pathway, junction elongation is a mechanical trigger that initiates RhoA activation through an influx of intracellular calcium and recruitment of p115RhoGEF. However, mechanisms that tune the level of RhoA activation and Myosin II contractility during the process remain uncharacterized. Here, we show that the scaffolding protein Anillin localizes to Rho flares and regulates RhoA activity and actomyosin contraction at flares. Knocking down Anilli

SUBMITTER: Craig Z 

PROVIDER: S-EPMC11601591 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature