The pentameric chloride channel BEST1 is activated by extracellular GABA.

ABSTRACT: Bestrophin 1 (BEST1) is chloride channel expressed in the eye, central nervous system (CNS), and other tissues in the body. A link between BEST1 and the principal inhibitory neurotransmitter γ-aminobutyric acid (GABA) has been proposed. The most appreciated receptors for extracellular GABA are the GABA_B G-protein coupled receptors and the pentameric GABA_A chloride channels, both of which have fundamental roles in the CNS. Here, we demonstrate that BEST1 is directly activated by GABA. Through functional studies and atomic-resolution structures of human and chicken BEST1, we identify a GABA binding site on the channel's extracellular side and determine the mechanism by which GABA binding induces opening of the channel's central gate. This same gate is activated by intra

SUBMITTER: Pant S 

PROVIDER: S-EPMC11601618 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature