Remoscope: a label-free imaging cytometer for malaria diagnostics.

ABSTRACT: Malaria diagnostic testing in high transmission settings remains a burden on healthcare systems. Here we present Remoscope, a portable automated imaging cytometer that scans fresh, unstained whole blood using a custom neural network on low-cost hardware. By screening up to two million red blood cells, Remoscope performs label-free quantitative stage-specific detection of Plasmodium falciparum (Pf) in 1-12 minutes without sample fixation, staining, or slide scanning. Flow is used to achieve high cellular throughput, with blood confined to a 4.5 μm monolayer in low-cost disposable flow cartridges. Remoscope performance was benchmarked in vitro by titration of cultured parasites into uninfected whole blood at concentrations of 17.1-710,000 parasites/μL. Counts generated b

SUBMITTER: Lebel PM 

PROVIDER: S-EPMC11601755 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature