Global coupling of R-loop dynamics with RNA polymerase II modulates gene expression and early development of Drosophila.

ABSTRACT: R-loops are involved in many biological processes in cells, yet the regulatory principles for R-loops in vivo and their impact on development remain to be explored. Here, we modified the CUT&Tag strategy to profile R-loops in Drosophila at multiple developmental stages. While high GC content promotes R-loop formation in mammalian cells, it is not required in Drosophila. In contrast, RNAPII abundance appears to be a universal inducing factor for R-loop formation, including active promoters and enhancers, and H3K27me3 decorated repressive regions and intergenic repeat sequences. Importantly, such a regulatory relationship is dynamically maintained throughout development, and development-related transcription factors may regulate RNAPII activation and R-loop dynamics. By ablating Spt6, we fur

SUBMITTER: Zhang X 

PROVIDER: S-EPMC11602159 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature