Synthetic translational coupling element for multiplexed signal processing and cellular control.

ABSTRACT: Repurposing natural systems to develop customized functions in biological systems is one of the main thrusts of synthetic biology. Translational coupling is a common phenomenon in diverse polycistronic operons for efficient allocation of limited genetic space and cellular resources. These beneficial features of translation coupling can provide exciting opportunities for creating novel synthetic biological devices. Here, we introduce a modular synthetic translational coupling element (synTCE) and integrate this design with de novo designed riboregulators, toehold switches. A systematic exploration of sequence domain variants for synTCEs led to the identification of critical design considerations for improving the system performance. Next, this design approach was seamlessly integrated into

SUBMITTER: Goh H 

PROVIDER: S-EPMC11602170 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature