Differential effects of prolonged post-fixation on immunohistochemical and histochemical staining for postmortem human brains.

ABSTRACT:

Purpose

Immunohistochemical (IHC) and histochemical (HC) staining techniques are widely used on human brains that are post-fixed in formalin and stored in brain banks worldwide for varying durations, from months to decades. Understanding the effects of prolonged post-fixation, postmortem interval (PMI), and age on these staining procedures is important for accurately interpreting their outcomes, thereby improving the diagnosis and research of brain disorders afflicting millions of people worldwide.

Methods

In this study, we conducted both IHC and HC staining on the prefrontal cortex of postmortem human brains post-fixed for 1, 5, 10, 15, and 20 years. For IHC staining, we used two antibodies for each marker: the neuron marker neuronal nuclear antigen (NeuN), the astrocyte ma

SUBMITTER: Ma W 

PROVIDER: S-EPMC11602276 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature