Production and purification of outer membrane vesicles encapsulating green fluorescent protein from <i>Escherichia coli</i>: a step towards scalable OMV technologies.

ABSTRACT: Outer membrane vesicles (OMVs) are spherical structures that contain a small fraction of the periplasm of Gram-negative bacteria, surrounded by its outer membrane. They are naturally produced and detached from the bacterial surface, participate in diverse biological processes, and their diameter size is in the range of 10-300 nm. OMVs have gained interest in different applications, such as the development of biosensors, vaccines, protein chips, and the encapsulation of heterologous proteins and peptides expressed by these microorganisms. However, the use of OMVs in these applications is limited due to the low yields and high purification costs. In this study, we produced green fluorescent protein (GFP) encapsulated into OMVs using Escherichia coli JC8031 transformed with pTRC99A-ssT

SUBMITTER: Torres-Vanegas JD 

PROVIDER: S-EPMC11602331 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature