The ubiquitous pyridoxal 5'-phosphate-binding protein is also an RNA-binding protein.

ABSTRACT: The pyridoxal 5'-phosphate binding protein (PLP-BP) is believed to play a crucial role in PLP homeostasis, which may explain why it is found in living organisms from all kingdoms. Escherichia coli YggS is the most studied homolog, but human PLP-BP has also attracted much attention because variants of this protein are responsible for a severe form of B₆-responsive neonatal epilepsy. Yet, how PLP-BP is involved in PLP homeostasis, and thus what its actual function is in cellular metabolism, is entirely unknown. The present study shows that YggS binds RNA and that the strength of this interaction is modulated by PLP. A key role in RNA binding is clearly played by Lys137, an invariant residue located on a protein loop away from the PLP binding site, whose importance has been highlig

SUBMITTER: Graziani C 

PROVIDER: S-EPMC11602438 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature