Clonal dynamics after allogeneic haematopoietic cell transplantation.

ABSTRACT: Allogeneic haematopoietic cell transplantation (HCT) replaces the stem cells responsible for blood production with those from a donor^1,2. Here, to quantify dynamics of long-term stem cell engraftment, we sequenced genomes from 2,824 single-cell-derived haematopoietic colonies of ten donor-recipient pairs taken 9-31 years after HLA-matched sibling HCT³. With younger donors (18-47 years at transplant), 5,000-30,000 stem cells had engrafted and were still contributing to haematopoiesis at the time of sampling; estimates were tenfold lower with older donors (50-66 years). Engrafted cells made multilineage contributions to myeloid, B lymphoid and T lymphoid populations, although individual clones often showed biases towards one or other mature cell type. Recipients had low

SUBMITTER: Spencer Chapman M 

PROVIDER: S-EPMC11602715 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature