Intracellular Calcium Dynamics in Primary Human Adrenocortical Cells Deciphered with a Novel Pipeline.

ABSTRACT:

Introduction

The fluctuations of the intracellular Ca²⁺ concentration ([Ca²⁺]_i) are key physiological signals for cell function under normal conditions and can undergo profound alterations in disease states, as high blood pressure due to endocrine disorders like primary aldosteronism (PA). However, when assessing such fluctuations several parameters in the Ca²⁺ signal dynamics need to be considered, which renders their assessment challenging.

Aim

Aim to develop an observer-independent custom-made pipeline to analyze Ca²⁺ dynamics in terms of frequency and peak parameters, as amplitude, full width at half maximum (FWHM) and area under the curve (AUC).

Methods

We applied a custom-made methodology to aldos

SUBMITTER: Ajjour H 

PROVIDER: S-EPMC11602809 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature