Parameter Estimation and Identifiability in Kinetic Flux Profiling Models of Metabolism.

ABSTRACT: Metabolic fluxes are the rates of life-sustaining chemical reactions within a cell and metabolites are the components. Determining the changes in these fluxes is crucial to understanding diseases with metabolic causes and consequences. Kinetic flux profiling (KFP) is a method for estimating flux that utilizes data from isotope tracing experiments. In these experiments, the isotope-labeled nutrient is metabolized through a pathway and integrated into the downstream metabolite pools. Measurements of proportion labeled for each metabolite in the pathway are taken at multiple time points and used to fit an ordinary differential equations model with fluxes as parameters. We begin by generalizing the process of converting diagrams of metabolic pathways into mathematical models composed of differ

SUBMITTER: Guppy B 

PROVIDER: S-EPMC11602815 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature