On the long-term storage of tissue for fluorescence and electron microscopy: lessons learned from rat liver samples.

ABSTRACT: Occasionally, tissue samples cannot be processed completely and are stored under varying conditions for extended periods. This is particularly beneficial in interinstitutional studies where a given research setting may lack the expertise or infrastructure for sample processing, imaging and data analysis. Currently, there is limited literature available on the controlled storage of biological tissues in primary fixatives for fluorescence and electron microscopy. In this contribution, we mimicked various tissue storage scenarios by taking different fixation conditions, storage temperatures and storage durations into account. Rat liver tissue was used for its well-known diversity of cellular ultrastructure and microscopy analysis. Fluorescent labelling of actin, DNA and lipids were employed i

SUBMITTER: Shami GJ 

PROVIDER: S-EPMC11602835 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature