The extracellular vesicle tetraspanin CD63 journey from the testis through the epididymis to mature bull sperm.

ABSTRACT: The important role of extracellular vesicles, which are considered key mediators of intercellular communication under physiological and pathological conditions, in various cellular processes, including those crucial for mammalian reproduction, has been increasingly studied. Tetraspanins, including CD63, are widely used as markers of extracellular vesicles, but they may also play a role in their biogenesis, cargo selection, cell targeting, and uptake. This study aimed to map the journey of the extracellular vesicle protein tetraspanin CD63 from the testis through the epididymis into mature bull sperm via an approach that included immunohistochemistry (immunofluorescence and immunoperoxidase staining), Western blot analysis, and immunoprecipitation analysis. We described the presence of CD63

SUBMITTER: Jankovicova J 

PROVIDER: S-EPMC11603341 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature