Transcriptome-wide mapping of internal mRNA N⁷-methylguanosine in sporulated and unsporulated oocysts of Eimeria tenella reveals stage-specific signatures.

ABSTRACT:

Background

Growing evidence indicates that N⁷-methylguanosine (m⁷G) modification plays critical roles in epigenetic regulation. However, no data regarding m⁷G modification are currently available in Eimeria tenella, a highly virulent species causing coccidiosis in chickens.

Methods

In the present study, we explore the distribution of internal messenger RNA (mRNA) m⁷G modification in sporulated and unsporulated oocysts of E. tenella as well as its potential biological functions during oocyst development using methylated RNA immunoprecipitation sequencing (MeRIP-seq) and mRNA sequencing (mRNA-seq), and the mRNA-seq and MeRIP-seq data were verified by the quantitative reverse transcription polymerase chain reaction (RT-qPCR) and MeRIP

SUBMITTER: Fan QX 

PROVIDER: S-EPMC11603632 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature