Antimicrobial resistance, virulence genes profiles and molecular epidemiology of carbapenem-resistant Klebsiella pneumoniae strains from captive giant pandas (Ailuropoda melanoleuca).

ABSTRACT:

Background

Carbapenem-resistant Klebsiella pneumoniae (CRKP) increases the difficulty of clinical treatment of giant pandas. This study aimed to investigate the antibiotic susceptibility, antibiotic resistance genes (ARGs), mobile genetic elements (MGEs), virulence genes, and molecular epidemiology of CRKP strains isolated from giant pandas. A total of 187 nonduplicated Klebsiella pneumoniae (KP) isolates were collected from fresh feces of captive giant pandas at the Chengdu Research Base of Giant Panda Breeding. Then CRKP were isolated and identified through carbapenase Carba NP assay. Subsequently, the antimicrobial susceptibility testing and antibiotic resistance genes of CRKP isolates were studied by disk diffusion (K-B) and HT-qPCR, respectively. Then both the MGEs and virulen

SUBMITTER: Yan X 

PROVIDER: S-EPMC11603722 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature