Highly efficient generation of mature megakaryocytes and functional platelets from human embryonic stem cells.

ABSTRACT:

Background

Platelet transfusion therapy has made a great breakthrough in clinical practice, and the differentiation of human embryonic stem cells (hESCs) to produce functional platelets has become a new potential approach, however, efficient generation of functional platelets still faces great challenges. Here, we presented a novel approach to highly and efficiently generate mature megakaryocytes (MKs) and functional platelets from hESCs.

Methods

In hypoxic conditions, we successfully replicated the maturation process of MKs and platelets in a controlled in vitro environment by introducing an optimal combination of cytokines at various stages of development. This method led to the generation of MKs and platelets derived from hESCs. Subsequently, mature MKs and functional pla

SUBMITTER: Chen C 

PROVIDER: S-EPMC11603724 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature