Development of a multiplex real-time PCR for the simultaneous detection of monkeypox virus clades I, II, and goatpox virus.

ABSTRACT:

Introduction

Monkeypox virus (MPXV) hosts are of multiple species, with a risk of cross-species transmission. This phenomenon poses a threat to unreported affected domestic animals and increases the risk to human public health. Clinical diagnostics continue to face challenges regarding specificity among poxviruses. The need for a rapid and precise assay to differentiate between MPXV clades I and II, as well as goatpox virus (GTPV) is essential for enhancing our capacity for disease prevention, control, and epidemiological investigation.

Methods

To address this need, we have successfully developed a multiplex real-time PCR assay targeting MPXV D14L gene for clade I, MPXV D18L gene for clade II, and GTPV RPO30 gene, which can simultaneously detect MPXV clades I and II as well

SUBMITTER: Lin Y 

PROVIDER: S-EPMC11603957 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature