Identification of serum N-glycans signatures in three major gastrointestinal cancers by high-throughput N-glycome profiling.

ABSTRACT:

Background

Alternative N-glycosylation of serum proteins has been observed in colorectal cancer (CRC), esophageal squamous cell carcinoma (ESCC) and gastric cancer (GC), while comparative study among those three cancers has not been reported before. We aimed to identify serum N-glycans signatures and introduce a discriminative model across the gastrointestinal cancers.

Methods

The study population was initially screened according to the exclusion criteria process. Serum N-glycans profiling was characterized by a high-throughput assay based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Diagnostic model was built by random forest, and unsupervised machine learning was performed to illustrate the differentiation between the thre

SUBMITTER: Liu S 

PROVIDER: S-EPMC11604001 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature