Whole-brain in situ mapping of neuronal activation in <i>Drosophila</i> during social behaviors and optogenetic stimulation.

ABSTRACT: Monitoring neuronal activity at single-cell resolution in freely moving Drosophila engaged in social behaviors is challenging because of their small size and lack of transparency. Extant methods, such as Flyception, are highly invasive. Whole-brain calcium imaging in head-fixed, walking flies is feasible but the animals cannot perform the consummatory phases of social behaviors like aggression or mating under these conditions. This has left open the fundamental question of whether neurons identified as functionally important for such behaviors using loss- or gain-of-function screens are actually active during the natural performance of such behaviors, and if so during which phase(s). Here, we perform brain-wide mapping of active cells expressing the Immediate Early Gene hr38

SUBMITTER: Watanabe K 

PROVIDER: S-EPMC11604218 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature