Time-dependent phenotypical changes of microglia drive alterations in hippocampal synaptic transmission in acute slices.

ABSTRACT: It is widely acknowledged that microglia actively regulate synaptic function in the brain. Remarkably, much of our understanding regarding the role of microglia in synaptic regulation is derived from studies in acute brain slices. However, it is still uncertain to what extent the preparation and maintenance of acute slices can influence microglial function and whether microglial changes may affect synaptic transmission. In this study, we examined the impact of acute slice resting time on hippocampal CA1 microglia, by assessing morphological and functional parameters at two distinct time intervals. We report that after 4 h from slicing microglia undergo morphological, functional, and transcriptional changes, including a decrease in the number of branches and in their movement speed. Further

SUBMITTER: Ferrucci L 

PROVIDER: S-EPMC11604457 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature