Implicating type 2 diabetes effector genes in relevant metabolic cellular models using promoter-focused Capture-C.

ABSTRACT:

Aims/hypothesis

Genome-wide association studies (GWAS) have identified hundreds of type 2 diabetes loci, with the vast majority of signals located in non-coding regions; as a consequence, it remains largely unclear which 'effector' genes these variants influence. Determining these effector genes has been hampered by the relatively challenging cellular settings in which they are hypothesised to confer their effects.

Methods

To implicate such effector genes, we elected to generate and integrate high-resolution promoter-focused Capture-C, assay for transposase-accessible chromatin with sequencing (ATAC-seq) and RNA-seq datasets to characterise chromatin and expression profiles in multiple cell lines relevant to type 2 diabetes for subsequent functional follow-up analyses: EndoC

SUBMITTER: Wachowski NA 

PROVIDER: S-EPMC11604697 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature