Investigating microstructural changes between in vivo and perfused ex vivo marmoset brains using oscillating gradient and b-tensor encoded diffusion MRI at 9.4 T.

ABSTRACT:

Purpose

To investigate microstructural alterations induced by perfusion fixation in brain tissues using advanced diffusion MRI techniques and estimate their potential impact on the application of ex vivo models to in vivo microstructure.

Methods

We used oscillating gradient spin echo (OGSE) and b-tensor encoding diffusion MRI to examine in vivo and ex vivo microstructural differences in the marmoset brain. OGSE was used to shorten effective diffusion times, whereas b-tensor encoding allowed for the differentiation of isotropic and anisotropic kurtosis. Additionally, we performed Monte Carlo simulations to estimate the potential microstructural changes in the tissues.

Results

We report large changes (˜50%-60%) in kurtosis frequency dispersion (OGSE) and in both anisotr

SUBMITTER: Santini T 

PROVIDER: S-EPMC11604852 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature