Quadrant darkfield for label-free imaging of intracellular puncta.

ABSTRACT: Imaging changes in subcellular structure is critical to understanding cell behavior but labeling can be impractical for some specimens and may induce artifacts. Although darkfield microscopy can reveal internal cell structures, it often produces strong signals at cell edges that obscure intracellular details. By optically eliminating the edge signal from darkfield images, we can resolve and quantify changes to cell structure without labeling. We introduce a computational darkfield imaging approach named quadrant darkfield (QDF) to separate smaller cellular features from large structures, enabling label-free imaging of cell organelles and structures in living cells. Using a programmable LED array as the illumination source, we vary the direction of illumination to encode additional informat

SUBMITTER: Moustafa TE 

PROVIDER: S-EPMC11605245 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature