Investigation of the free radical scavenging ability of l-tryptophan and its derivatives using experimental methods and quantum chemical calculations.

ABSTRACT: The free radical scavenging ability of l-tryptophan (LP) and 5-hydroxy-l-tryptophan (HLP) was evaluated using experimental and theoretical methods. The impact of antioxidant concentration on the scavenging of DPPH˙ and ABTS˙+ free radicals was assessed for both compounds. The results indicated that HLP exhibited superior scavenging ability, with IC50 values of 31.96 × 10-7 ± 0.85 × 10-7 M for DPPH˙ and 8.69 × 10-6 ± 0.95 × 10-6 M for ABTS˙+. In contrast, LP showed higher IC50 values of 9.51 × 10-3 ± 0.53 × 10-3 M for DPPH˙ assay and 8.91 × 10-4 ± 0.73 × 10-4 M for ABTS˙+ assay, indicating less effective scavenging. Theoretical calculations, performed by analyzing frontier molecular orbitals and molecular electrostatic potential, revealed that electron-donating regions were primarily distri

SUBMITTER: Quy Huong D 

PROVIDER: S-EPMC11605380 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature