Monocyte-derived extracellular vesicles, stimulated by Trypanosoma cruzi, enhance cellular invasion in vitro via activated TGF-β1.

ABSTRACT: During cell invasion, large Extracellular Vesicle (lEV) release from host cells was dose-dependently triggered by Trypanosoma cruzi metacyclic trypomastigotes (Mtr). This lEV release was inhibited when IP₃-mediated Ca²⁺ exit from the ER and further Ca²⁺ entry from plasma membrane channels was blocked, but whilst any store-independent Ca²⁺ entry (SICE) could continue unabated. That lEV release was equally inhibited if all entry from external sources was blocked by chelation of external Ca²⁺ points to the major contributor to Mtr-triggered host cell lEV release being IP₃/store-mediated Ca²⁺ release, SICE playing a minor role. Host cell lEVs were released through Mtr interaction with host cell lipid raft domains, int

SUBMITTER: Ansa-Addo EA 

PROVIDER: S-EPMC11605483 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature