Reproducible generation of human liver organoids (HLOs) on a pillar plate platform <i>via</i> microarray 3D bioprinting.

ABSTRACT: Human liver organoids (HLOs) hold significant potential for recapitulating the architecture and function of liver tissues in vivo. However, conventional culture methods of HLOs, forming Matrigel domes in 6-/24-well plates, have technical limitations such as high cost and low throughput in organoid-based assays for predictive assessment of compounds in clinical and pharmacological lab settings. To address these issues, we have developed a unique microarray 3D bioprinting protocol of progenitor cells in biomimetic hydrogels on a pillar plate with sidewalls and slits, coupled with a clear bottom, 384-deep well plate for scale-up production of HLOs. Microarray 3D bioprinting, a droplet-based printing technology, was used to generate a large number of small organoids on the pillar plate

SUBMITTER: Shrestha S 

PROVIDER: S-EPMC11605706 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature