Nuclear release of eIF1 restricts start-codon selection during mitosis.

ABSTRACT: Regulated start-codon selection has the potential to reshape the proteome through the differential production of upstream open reading frames, canonical proteins, and alternative translational isoforms^1-3. However, conditions under which start codon selection is altered remain poorly defined. Here, using transcriptome-wide translation-initiation-site profiling⁴, we reveal a global increase in the stringency of start-codon selection during mammalian mitosis. Low-efficiency initiation sites are preferentially repressed in mitosis, resulting in pervasive changes in the translation of thousands of start sites and their corresponding protein products. This enhanced stringency of start-codon selection during mitosis results from increased association between the 40S ribosom

SUBMITTER: Ly J 

PROVIDER: S-EPMC11605796 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature