Engineering the secretome of Aspergillus niger for cellooligosaccharides production from plant biomass.

ABSTRACT:

Background

Fermentation of sugars derived from plant biomass feedstock is crucial for sustainability. Hence, utilizing customized enzymatic cocktails to obtain oligosaccharides instead of monomers is an alternative fermentation strategy to produce prebiotics, cosmetics, and biofuels. This study developed an engineered strain of Aspergillus niger producing a tailored cellulolytic cocktail capable of partially degrading sugarcane straw to yield cellooligosaccharides.

Results

The A. niger prtT∆ strain created resulted in a reduced extracellular protease production. The prtT∆ background was then used to create strains by deleting exoenzyme encoding genes involved in mono- or disaccharide formation. Consequently, we successfully generated a tailored prtT∆bglA∆ strain by eliminati

SUBMITTER: de Figueiredo FL 

PROVIDER: S-EPMC11606201 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature