Impact of mesenchymal stem cell size and adhesion modulation on in vivo distribution: insights from quantitative PET imaging.

ABSTRACT:

Background

Successful engraftment and localization of mesenchymal stem cells (MSCs) within target tissues are critical factors influencing their therapeutic efficacy for tissue repair and regeneration. However, the relative contributions of biophysical factors like cell size and adhesion capacity in regulating MSC distribution in vivo remain incompletely understood.

Methods

Cell adhesion peptides and hanging drop method were used to modify the adhesive capacity and size of MSCs. To quantitatively track the real-time biodistribution of transplanted MSCs with defined size and adhesion profiles in living mice and rats, the non-invasive positron emission tomography (PET) imaging was applied.

Results

Surface modification with integrin binding peptides like RGD, GFOGER, and

SUBMITTER: Ji X 

PROVIDER: S-EPMC11606219 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature