Observation of E-cadherin adherens junction dynamics with metal-induced energy transfer imaging and spectroscopy.

ABSTRACT: Epithelial cadherin (E-cad) mediated cell-cell junctions play a crucial role in the establishment and maintenance of tissues and organs. In this study, we employed metal-induced energy transfer imaging and spectroscopy to investigate variations in intermembrane distance during adhesion between two model membranes adorned with E-cad. By correlating the measured intermembrane distances with the distinct E-cad junction states, we probed the dynamic behavior and diversity of E-cad junctions across different binding pathways. Our observations led to the identification of a transient intermediate state referred to as the X-dimeric state and enabled a detailed analysis of its kinetics. We discovered that the formation of the X-dimer leads to significant membrane displacement, subsequently impacti

SUBMITTER: Chen T 

PROVIDER: S-EPMC11606943 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature