The impact of mechanical tuning on the printability of decellularized amniotic membrane bioinks for cell-laden bioprinting of soft tissue constructs.

ABSTRACT: Decellularized extracellular matrix (dECM) bioinks hold significant potential in the 3D bioprinting of tissue-engineered constructs (TECs). While 3D bioprinting allows for the creation of custom-designed TECs, the development of bioinks based solely on dAM, without the inclusion of supporting agents or chemical modifications, remains underexplored. In this study, we present the concentration-dependent printability and rheological properties of dAM bioinks, along with an analysis of their in vitro cellular responses. Our findings demonstrate that increasing dAM concentrations, within the range of 1 to 3% w/v, enhances the mechanical moduli of the bioinks, enabling the 3D printing of flat structures with superior shape fidelity. In vitro assays reveal high cell viability across all dAM bioin

SUBMITTER: Kafili G 

PROVIDER: S-EPMC11606975 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature